Journal: Frontiers in Endocrinology

Article Title: Ultrasound combined with microbubbles promotes diabetic wound healing by regulating macrophage polarization

doi: 10.3389/fendo.2026.1781906

Figure Lengend Snippet: Immunohistochemical staining of wounds in diabetic rats. (A) Representative images of Ki67 immunohistochemical staining in healing tissues from three experimental groups; (B) Quantitative analysis of Ki67-positive cells across groups; (C) Immunohistochemical staining of CD31 in diabetic wounds; (D) Statistical results of CD31-positive expression. Data are presented as mean ± SD (n = 4); *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: After three washes with PBS, they were incubated overnight at 4 °C with anti-CD31 antibodies (1:1000, abcam) and anti-Ki67 antibodies (1:1000, Proteintech).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Cell stem cell

Article Title: Melanocyte Stem Cell Activation and Translocation Initiate Cutaneous Melanoma in Response to Ultraviolet Exposure

doi: 10.1016/j.stem.2017.09.001

Figure Lengend Snippet: (A) MCSC translocation by topical TPA treatment shown by tdTomato lineage tracing. (B) Experimental scheme. (C) Macroscopic phenotypes in TBP mice with/without topical TPA treatment, and quantification of epidermal pigmentation. n = 10 TPA-treated control, n = 7 non-TPA TBP and n = 7 TPA-treated TBP mice. (D) Histological phenotypes of TPA-induced melanoma during quiescent MCSC, telogen period, and co-immunostaining for Sox10, a melanocyte stem cell/melanoma marker, with Ki67, a marker of proliferation. (E) Experimental scheme. Dex., dexamethasone. (F) Relative number of MCSC migration was quantified via tdTomato lineage tracing in TT mice, compared to the total number of hair follicles (≥ 500 hair follicles / each). Mean ± SEM, n = 10 / group. (G) Experimental scheme. (H) Macroscopic phenotypes with the quantification of epidermal pigmentation. n = 6 low-dose (80 mJ cm−2) UVB, n = 10 per each group (UVB only or UVB + Dex.). Data are represented as Mean ± SEM. Also see Figures S5 and S6.

Article Snippet: Rabbit monoclonal anti-Ki-67 , Rockland , Cat#900-C01-B38; RRID:AB_2142230.

Techniques: Translocation Assay, Immunostaining, Marker, Migration

Journal: Cell stem cell

Article Title: Melanocyte Stem Cell Activation and Translocation Initiate Cutaneous Melanoma in Response to Ultraviolet Exposure

doi: 10.1016/j.stem.2017.09.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-Ki-67 , Rockland , Cat#900-C01-B38; RRID:AB_2142230.

Techniques: Plasmid Preparation, Recombinant, DNA Ligation, SYBR Green Assay, BIA-KA, Ab Array, Software, CRISPR

Journal: Frontiers in Molecular Biosciences

Article Title: Antimetastatic and antitumor activities of oncolytic NDV AMHA1 in a 3D culture model of breast cancer

doi: 10.3389/fmolb.2024.1331369

Figure Lengend Snippet: Analysis of the expressions of caspase-3, P21, and Ki67 by immunofluorescence assay in the 3D spheroids of the breast cancer metastasis model and normal tissue spheroids. Spheroids were infected with NDV (MOI 10) for 24 h; the spheroids were stained with anti-caspase-3, anti-P21, and anti-KI67 antibodies. Caspase-3 and P21 proteins exhibit a high expression of green fluorescence, while there is no expression in untreated spheroids (control). Furthermore, KI67 was absent in cancer spheroids treated with NDV. Normal tissue spheroids (hATMSCs) were not affected by the presence of virus as there were no notable changes in the expression of these proteins between treated and untreated spheroids, again confirming the safety of the virus.

Article Snippet: Cells were then treated with 1 μgmL −1 of each of the primary markers, KI67 (mouse monoclonal antibody, dilution 1:200; Elabscience, China), caspase-3 (mouse monoclonal antibody, dilution 1:200; Elabscience, China), and P21 (mouse monoclonal antibody, dilution 1: 500, Santa Cruz, CA, United States), which were diluted in blocking buffer and incubated for 1:30 h or overnight at 4°C.

Techniques: Immunofluorescence, Infection, Staining, Expressing, Fluorescence, Control, Virus