Journal: Cancer Research Communications

Article Title: Novel Syngeneic Cell Lines for Studying High-Risk BRAF V600E -Driven Colorectal Cancer In Vivo

doi: 10.1158/2767-9764.CRC-25-0599

Figure Lengend Snippet: All NaJa cell lines successfully engraft into syngeneic immunocompetent C57BL/6N mice and recapitulate their individual 2D morphologies in vivo . A, Schematic overview of the PVI of NaJa cells ( n = 2 NaJa-D, n = 10 NaJa-F, and n = 3 NaJa-G). B, Left, livers showing metastatic nodules 4 weeks after injection (black arrows) and invasive growth in the pancreatic tissue (blue circles). Scale bar, 1 cm. Hematoxylin and eosin: Histology showing that all NaJa cell lines establish tumors (pale blue) in the liver parenchyma (pink) with a differentiation state matching their epithelial or mesenchymal in vitro morphology. Right, IHC and immunofluorescence (IF) staining of proliferation marker Ki-67 (left) and intestinal differentiation markers CDX2 (middle) and E-cadherin (right). L, liver; T, tumor. Scale bar (overview), 500 μm, scale bar (zoom, IHC, and IF), 50 μm. C, Quantification of Ki-67 and CDX2 IHC staining in NaJa cell–induced liver metastases. Each dot represents an individual tumor; different shapes indicate individual tissue sections, and colors denote individual mice. Data are shown as the median ±95% confidence interval (CI).Statistical significance was assessed using the Kruskal–Wallis test followed by Dunn multiple comparison test. **, P < 0.01; ****, P < 0.0001. D, IHC staining of ERK and its downstream target DUSP6. Red arrows indicate examples of nuclear ERK staining. L, liver; T, tumor. Scale bar, 50 μm. [ A, Created by J. Traichel in BioRender. Brummer, T. (2025) https://BioRender.com/8ogg64c .]

Article Snippet: The formalin-fixed, paraffin-embedded (FFPE) sections underwent standard processing and staining procedures using the following primary antibodies directed against E-cadherin (1:50, #610181, BD Laboratories; RRID: AB_397580) and Ki-67 (1:400, #9129, Cell Signaling Technology; RRID: AB_2687446).

Techniques: In Vivo, Injection, In Vitro, Immunofluorescence, Staining, Marker, Immunohistochemistry, Comparison

Journal: STAR Protocols

Article Title: Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials

doi: 10.1016/j.xpro.2022.101362

Figure Lengend Snippet:

Article Snippet: Anti-Ki-67 (B56)-168Er—100 Tests (1:100) , Fluidigm , Cat#3168007B.

Techniques: Recombinant, Saline, Staining, Blocking Assay, Isolation, Software, Cell Counting, Cytometry

Journal: NPJ Breast Cancer

Article Title: Targeting GD2 with naxitamab overcomes GD3 synthase-driven immune suppression in triple-negative breast cancer

doi: 10.1038/s41523-025-00843-7

Figure Lengend Snippet: A Analysis of GD3S ( ST8SIA1 ) mRNA expression in METABRIC and TCGA (pan cancer) BC patient datasets shows significant correlation with immune checkpoint inhibitors. In the METABRIC dataset, Pearson’s correlation coefficients indicate significant correlations of GD3S mRNA expression with CTLA-4 ( r = 0.4; P < 0.001), LAG3 ( r = 0.33; P < 0.001), PD1 ( r = 0.3; P < 0.001), CD25 ( r = 0.38; P < 0.001), and SIRPα ( r = 0.36; P < 0.001). Similarly, in the TCGA dataset, GD3S mRNA expression was significantly correlated with CTLA-4 ( r = 0.47; P < 0.001), LAG3 ( r = 0.33; P < 0.001), PD1 ( r = 0.38; P < 0.001), CD25 ( r = 0.45; P < 0.001), and SIRPα ( r = 0.56; P < 0.001). B Representative IHC images obtained by sequential multiplex immunofluorescence images (Lunaphore COMET) from FFPE samples of TNBC patients with high or low GD3S expression ( n = 20 total) were used to compare immune infiltration patterns, showing distinct fluorescence intensities for PANK, Ki67, CD163, CD86, CD56, CD8, CD4, FOXP3, and GD3S within tumor tissues. C H-scores for GD3S expression in BC patients categorized into high and low GD3S expression groups ( ****P < 0.0001). Immune cell counts using Visiopharm IHC image analysis software show differences in the expression of CD8, CD4, CD86, and CD56 between the high and low GD3S expression groups ( ****P < 0.0001; **P < 0.01). Scale bar in IHC images represents 55 µm with 20x magnification. Bar graphs display means ± standard deviations.

Article Snippet: We also purchased the following antibodies: pan-Cytokeratin (Proteintech, cat. #26411-1-AP, rabbit host) along with GD3S (Proteintech, cat. #24918-1-AP, rabbit host), CD45 (Fortislife, clone BL-178-1267, cat. #A700-012, rabbit host), Ki67 (Bethyl, clone BLR021E, cat. #A700-021CF, rabbit host), CD3 (Fortislife, clone BL-298-5012, cat. #A700-016F, rabbit host), CD8 (Fortislife, clone c8/144B, cat. #A500-012A, mouse host), CD4 (Bethyl, clone BL-155-1C11, cat. #A700-015, rabbit host), FOXP3 (Bethyl, clone BLR034F, cat. #A700-034, rabbit host), CD68 (Fortislife, cat. #A500-018ACF, mouse host), CD163 (Invitrogen, clone I0D6, cat. #MA5-11458, mouse host), CD86 (CST, clone E2G8P, cat. #76755SF, rabbit host), CD56 (Fortislife, clone BLR152J, cat. #A700-152, rabbit host) and human IgG1 Kappa antibody (hIgG, cat. #0151K-14, SouthernBiotech).

Techniques: Expressing, Multiplex Assay, Immunofluorescence, Fluorescence, Software

Journal: Archives of Gynecology and Obstetrics

Article Title: rHGF interacts with rIGF-1 to activate the satellite cells in the striated urethral sphincter in rats: a promising treatment for urinary incontinence?

doi: 10.1007/s00404-018-4930-2

Figure Lengend Snippet: In Ki-67 staining, all nuclei were stained red with PI. The satellite cells expressing Ki67 were stained blue with Ki67 antibody. In MYH3 and NCAM staining, all nuclei were stained blue with DAPI. The satellite cells were stained red with MYH3 and NCAM antibody. All of the images are merged and show co-localization

Article Snippet: To detect Ki67, after cultivated with BSA for 45 min, the sections were incubated overnight at 4 °C with the Ki67 antibody (CST; 12075S), Propidium bromide (PI) was applied to the sections for 30 min to identify nuclei.

Techniques: Staining, Expressing

Journal: Frontiers in Endocrinology

Article Title: Ultrasound combined with microbubbles promotes diabetic wound healing by regulating macrophage polarization

doi: 10.3389/fendo.2026.1781906

Figure Lengend Snippet: Immunohistochemical staining of wounds in diabetic rats. (A) Representative images of Ki67 immunohistochemical staining in healing tissues from three experimental groups; (B) Quantitative analysis of Ki67-positive cells across groups; (C) Immunohistochemical staining of CD31 in diabetic wounds; (D) Statistical results of CD31-positive expression. Data are presented as mean ± SD (n = 4); *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: After three washes with PBS, they were incubated overnight at 4 °C with anti-CD31 antibodies (1:1000, abcam) and anti-Ki67 antibodies (1:1000, Proteintech).

Techniques: Immunohistochemical staining, Staining, Expressing